Equal-Tempered Open Guitar String(s) Tuning Tones: (1) E3 164.814 Hz (2) A3 220.000 Hz (3) D4 293.665 Hz (4) G4 391.995 Hz (5) B4 493.883 Hz (6) E5 659.255 Hz As any good musician knows, Bach tuned his Clavier by slightly lowering each note of the scale compromising the natural harmonics for the ability to play in every key with only a slight perception of dissonance in any particular key. This compromise has disillusioned many of a guitar player in my time. As when most people begin to learn guitar they first learn to tune their guitar by the major fifths (low E fifth fret to A etc., except G forth fret to B, or perhaps even the other way down high E seventh fret to B, B eighth fret to G etc.) Although the fifth method is some what flawed in itself, short of using an electronic or strobe tuner it is probably the best bet, at least you will be in tune with yourself. Then someone shows them harmonic tuning one day and they are messed up for life, stuck in that one key rut (generally harmonic tuning is done using the seventh fret harmonics, except the B string which is tuned to the G fifth fret harmonic, and vice a versa E fifth fret on the way down). If this brief explanation throws you off a bit, remember all octives are exactly doubled, with absolutely no audible beats between them. As for all the rest of the notes of any scale, they have beats because they are all slightly out of tune on purpose, so keep them like that, that is the way they are supposed to be. Do not argue with Bach, or else. Harmonic tuning does have its place in music, but generally it is the exception, not the rule. So be safe and stick to a good Equal-Tempered Guitar Tuning. The rest of the band will be glad you did. Sure I could go in to my whole tuning dissertation here but this time I will spare you. If you are truly interested in the physics and mathematics of the tuning and why we use the diatonic comma, do so research, or come listen to my lecture sometime: "Bach and Tonic!" Reference: The equal-tempered scale of western music is based upon an octave (frequency doubling) that contains 12 notes, or "semitones", each of which is 1.059463 (the 12th root of 2) times the one below it. The standard given 'American Reference Note' is A4 440 Hz. Included in this zip file is the "tune-guitar-sin.mp3" provided by KimDara.com's Studio Reference Lab in Austin, Texas using the above mentioned frequencies with a standard sine wave modulator and duration of five seconds each. tune-guitar-sin.mp3 File Size 470 KB Total running time 30 seconds.